Tufts CMS Rodent Importation Request

Animal Importation Form

Please complete this form if you are requesting animals from a source other than a Tufts CMS approved vendor. Contact TuftsCMS-importation@tufts.edu for any questions. Please read the Guidelines for Rodent Importation for information on categories of quarantine, costs, topical selamectin treatment and fenbendazole and uniprim antibiotic treatment given the animals on arrival, length of time in quarantine, and other important information.

Tufts Information

PI Name*

PI email deleted questtion. HIDE

PI Email*

Name of person completing this form*

deleted question. HIDE

Email of person completing this form*

Lab contact name to be written on cage cards*

Lab contact phone number to be written on cage cards*

Additional lab contact email (optional)

IACUC Protocol #*

The IACUC Pain and Distress Category for the imported mice will be:*

All Category CAll Category DAll Category EA combination of categories (we will follow up by email to match cage cards with categories)

Billing Account #*

Genotyping, Genetic Monitoring, and Microbiome

Tufts CMS strongly recommends that you genotype the animals you receive.

Transnetyx is offering a “Passport Program” which helps researchers eliminate variability and achieve better, more reproducible science by providing genotyping, genetic monitoring, and microbiome testing of mouse models at the time of importation (or after). The cost of the service is a flat fee of $250. Free access to Transnetyx Colony software for colony management also is included. If you are interested in this service, contact TuftsCMS-importation@tufts.edu and we can provide you with the hard copy Passport booklet from Transnetyx that contains unique QR codes as well as the 96-well plates for genotyping and the microbiome kits. We can assist you in collecting samples during importation quarantine or you can collect and submit the samples yourself following quarantine. For more information on the program, please contact the Transnetyx representative Karen Lindstrom at klindstrom@transnetyx.com.

Transnetyx Passport Program

I would like to receive a free Passport booklet (no obligation to use the service).

Source Information

Institution Name*

City

State

Country

PI name

PI email

Lab Contact email

Animal Facility Contact:

Please list the name and email of at least one person such as a veterinarian or animal export coordinator in the source animal facility who is knowledgeable about the facility health status and whom we can contact for health reports. This is generally NOT someone from the research laboratory. We will send this person a request for a health report.

Health Contact Name

Health Contact email*

Other contacts:

Please list emails (separated by a semicolon) for anyone else besides those contacts already listed who should be included on correspondence regarding this shipment

Animals

Species*

MouseRatOther

List all strains/stock/genetic mutations*

estimated # females*

estimated # males*

estimated total # cages*

Check one*

these are genetically engineered animals from a noncommercial vendor and will be bred here.the animals are not genetically engineered, or they will not be bred here

IACUC and IBC

A new IACUC amendment or IBC registration may be needed for the animals you are importing. Please review the following documents:

IBC Policy on the Use of Transgenic Animals

IACUC Policy Regarding Intraspecies Amendments

Contact IBC-office@tufts.edu or IACUC-office@tufts.edu for any questions.

Please check one

I plan to breed these rodents from a noncommercial source. The use of these genetically modified rodents is already described in the above listed IACUC protocol [Section V(c) or by amendment].I plan to breed these genetically engineered animals from a noncommercial source. The animals were not described in my IACUC protocol, but I have completed the IBC Genetically Modified Rodent Usage form for these animals.I plan to breed these genetically engineered rodents created by a noncommercial vendor, but the use of these animals is not described in my IACUC protocol or by IBC notification. I understand that IBC notification is required by completing the Genetically Modified Rodent Usage Form.Other

Check all that apply*

the animals have a known immunodeficiencythe animals have a phenotype that causes illness, abnormalities, or early deaththe animals require sterile caging (at additional cost)the animals require special husbandry such as special diets, medical treatments, etc.none of the above apply

STERILE CAGING: Standard quarantine caging is the same clean caging used in standard Tufts CMS animal housing rooms. If the animals have an immunodeficiency and you feel they would be better housed in special sterile caging in which the cages are autoclaved as individual units after cleaning, we can arrange this at additional charge. Sterile caging is meant to reduce the opportunistic organisms that the animals may encounter, but does not guarantee that the animals could become or do not arrive already infected with opportunistic agents.

Describe the immunodeficiency*

Describe the need for special caging, diets, or medical treatments*

Describe phenotypes with severe illness, abnormalities, or early death*

Planned Use

Please select ALL THAT APPLY for this importation*

some or all animals will be used immediately on arrival for terminal same-day experiments. The animals will not require housing.some or all animals will be housed in quarantine for up to 4 weeks for a terminal studysome or all of the animals will be housed in quarantine for 4 weeks or more for a terminal studysome or all of the animals will be housed in standard quarantine for 3 weeks and then will be transferred to a regular housing roomThese animals will not be quarantined because they come from an approved vendor. Tufts CMS importation staff will house the animals directly in a regular housing room on arrivalOther

Is this short-term terminal experiment an MCRI study?*

Yes, this is an MCRI studyNo

Is this long-term terminal experiment an MCRI study?*

Yes, this is an MCRI studyNo

Please check that you have read the following regarding terminal quarantine studies in MCRI*

FOR PROCEDURES IN THE MCRI LAB THAT DO NOT REQUIRE MCRI HOUSING, animals will be housed on arrival in acute quarantine. All animals will receive topical selamectin. Some health testing may be required on arrival depending on the health status of the facility of origin and the experimental plans. FOR STUDIES THAT REQUIRE HOUSING IN MCRI, A limited health testing program of serology and PCR will be done and billed to your lab. The results take 7-10 business days to obtain. If the results are negative, the animals can be brought to MCRI for housing in the satellite housing room.

Please check that you have read the following regarding immediate terminal use of animals:*

Animals that will be sacrificed on the day of arrival will be shipped to the main Tufts CMS facility and can be picked up there for transport to your lab in the original shipping crates. If your plans change, the mice can be housed for up to 28 days in acute quarantine until you are ready to use them. Please notify TuftsCMS-importation@tufts.edu if you do require housing for some or all of the animals.

Please check that you have read the following regarding quarantine of up to 4 weeks*

Animals housed for up to 4 weeks (28 days) post arrival will be given a dose of topical selamectin on arrival. Some health testing may be required on arrival depending on the health status of the facility of origin and the experimental plans. ANIMALS THAT REMAIN AFTER 28 DAYS WILL BE SWITCHED TO THE STANDARD QUARANTINE ROUTE WHICH INCLUDES HEALTH TESTING AT ADDITIONAL COST.

Please check that you have read the following regarding quarantine housing for more than 4 weeks for terminal experiment*

Animals that will be housed for a terminal experiment for more than 28 days will undergo the standard quarantine health testing procedures on arrival. If animals are here for more than 3 weeks, they can be moved out of quarantine if they pass the health testing. All animals will be given topical selamectin.

Please check that you have read the following regarding quarantine housing for more than 4 weeks for breeding colony establishment*

Animals will undergo the standard quarantine health testing procedures on arrival. Breeding can be set up after acclimation (about one week after arrival). Animals will be moved out of quarantine in about 3 weeks if they pass the health testing. All animals will be given topical selamectin on arrival.

The housing and use of imported animals presents some risk of spread of rodent pathogens to other Tufts CMS rodent colonies if the animals are not tested for common adventitious agents, which is the situation for animals used immediatedly on arrival or animals housed for less than 4 weeks. Reduce risk of cross contamination by following the steps below.

Please CHECK ALL BOXES to acknowledge the following requirements for use of animals in acute quarantine. *

Enter the quarantine room LAST during a day. After entering a quarantine room, you cannot enter another housing room for the rest of the day.To remove animals from acute quarantine to a lab, place the animals in clean cages, spray the outer cage surfaces with disinfectant, and transport the animals in brown paper bags to the lab.Do not have animals from acute quarantine and animals from the main facility in the lab at the same time (avoid the potential for cross-contamination).Disinfect all surfaces after lab procedures. You can obtain disinfectant from the Tufts CMS husbandry office. Sterilize or autoclave instruments after use.Bring empty cages back to the acute quarantine housing area and bag them in autoclave bags.

Briefly describe the experiment timeframe (anticipated start date and duration of study), brief summary of procedures, and location of procedures.*

List names of anyone who will need security card access to the quarantine room

Notes to the Tufts CMS importation staff:

When you have completed the form, hit the "Submit" button below. You will receive a notification of receipt of your form by email within a few minutes. If you do not, please contact TuftsCMS-importation@tufts.edu to ensure that the submission was received.