QFAGE cell engineering service EOI form

QFAGE cell engineering service EOI form

The University of Queensland, Australia
Name *
Email *
Laboratory *
Institute or company *
University
Phone Number
Project type *

Gene Knockout Genomic deletion Mutation (point mutation, small insertion or deletion) Knock-in (reporter, epitope tag, LoxP etc. ) Stable over-expression cell line Other or unknown
Service type *

CRISPR Cell pool CRISPR Cell clone Genotyping Other or unknown
Is it for grant application

Yes No
When do you want to start your project?

Month Day Year
Target and cell line information
Are you working with non-commercial cells such as in-house generated human primary cells or cell lines? *

YES NO
Cell line name and or ATCC # *
Species *
Cell-line Karyotype information *
Ploidy number
Target Gene name/locus, NCBI number *
Database link
Link to NCBI Gene, UCSC Genome Browser etc
Desired modification
specific mutation, knock-in sequence
Number of allele(s) or gene copy number to be modified *

1 2 3 4 or more unknown
Transfection method and efficiency
Will the modification of the gene negatively affect the survival or growth of your cell?

Yes No Unknown
Can your cell line be expanded from a single cell?

Yes No Not tested yet.
Is your stock of cell mycoplasma tested?

Yes Not yet
Please tell us more about your project
(eg. aim of project, details of modifications, etc, please provide some details to help us better understand your project)
Additional information
Do you have experience for single cell cloning?

Yes No
Have you done any Knockdown (siRNA or shRNA) assay on your target gene in your cell-line before?

Yes Not yet
Have you validate the expression of your target in your cell-line using qPCR/ELISA/western-blotting before?

Yes Not yet
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QFAGE cell engineering service EOI form

Target and cell line information

Additional information